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172 1011 actin n antibody  (Bio-Rad)


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    Bio-Rad 172 1011 actin n antibody
    172 1011 Actin N Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 5145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/172+1011+actin+n+antibody/Goat+Anti-Mouse+IgG+(H+%2B+L)-HRP+Conjugate/pm40440176-24-224-251
    Average 99 stars, based on 5145 article reviews
    172 1011 actin n antibody - by Bioz Stars, 2026-09
    99/100 stars

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    Recombinant:

    Article Title: Protocol for detecting SnRK2 kinase activity in plants by immunoblotting, in-gel assay, band shift, and immunoprecipitation.
    Article Snippet: Chemicals, peptides, and recombinant proteins Sodium hypochlorite (NaClO) Sinopharm Chemical Reagent CAS#: 7681-52-9 75% alcohol General reagent CAS#: G73537W Murashige & Skoog (MS) basal salt mixture Phyto Technology Cat#: M524 Sucrose Sinopharm Chemical Reagent CAS#: 57-50-1 Agar Sigma-Aldrich CAS#: 9002-18-0 NaCl Sinopharm Chemical Reagent CAS#: 7647-14-5 ABA Sinopharm Chemical Reagent CAS#: 14375-45-2 Mannitol Sinopharm Chemical Reagent CAS#: 69-65-8 Tris Sinopharm Chemical Reagent CAS#: 77-86-1 Sodium dodecyl sulfate (SDS) Sigma-Aldrich CAS#: 151-21-3 Glycine Sigma-Aldrich CAS#: 56-40-6 HEPES Sigma-Aldrich CAS#: 7365-45-9 EDTA Sinopharm Chemical Reagent CAS#: 60-00-4 EGTA Sigma-Aldrich CAS#: 67-42-5 Na3VO4 Sigma-Aldrich CAS#: 13721-39-6 NaF Sigma-Aldrich CAS#: 7681-49-4 β-glycerophosphate disodium Sigma-Aldrich CAS#: 154804-51-0 DTT Sigma-Aldrich CAS#: 3483-12-3 Leupeptin Sigma-Aldrich CAS#: 103476-89-7 Antipain Sigma-Aldrich Cat#: 10791 Aprotinin Roche Cat#: 10981532001 PMSF Sigma-Aldrich CAS#: 9036-06-0 HCl Sinopharm Chemical Reagent CAS#: 7647-01-0 MgCl2 Sigma-Aldrich CAS#: 7786-30-3 Histone type III-S Sigma-Aldrich CAS#: 9064-47-5 Triton X-100 Sigma-Aldrich CAS#: 9036-19-5 β-mercaptoethanol Sigma-Aldrich CAS#: 60-24-2 Color PAGE gel rapid preparation kit Epizyme Cat#: PG-112 Blue Plus IV protein marker TransGen Cat#: DM131-01 Trichloroacetic acid (TCA) Sigma-Aldrich CAS#: 76-03-9 Sodium pyrophosphate (NaPPi, Na4P2O7) Sigma-Aldrich CAS#: 13472-36-1 PVDF transfer membrane, 0.45 μm Invitrogen Cat#: 88518 Methanol Sinopharm Chemical Reagent Cat#: 67-56-1 Western blot fast stripping buffer Epizyme Cat#: PS107 Goat anti-rabbit IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1019 Anti-Myc tag (mouse monoclonal IgG1) Merck/Millipore Cat#: 05-724 Goat anti-mouse IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1011 ACTIN (N) antibody, rabbit polyclonal Abicode Cat#: R3772-1P Anti-GFP monoclonal antibody (JL-8) Takara Cat#: 632381 Anti-phospho-S175-SnRK2s antibody ABclonal Cat#: AP1481 Quick Start Bradford 1x dye reagent Bio-Rad Cat#: 5000205 Experimental models: Organisms/strains Arabidopsis thaliana: Col-0 N/A Arabidopsis thaliana: 35Spro:SnRK2.1-YFP in Col-0 background N/A Arabidopsis thaliana: SnRK2.4pro:SnRK2.4-GFP in snrk2.1/4/5/7/8/9/10 mutant background N/A Software and algorithms Amersham Typhoon scanner Amersham Typhoon N/A (Continued on next page) 2 STAR Protocols 6, 103842, June 20, 2025

    Polyacrylamide Gel Electrophoresis:

    Article Title: Protocol for detecting SnRK2 kinase activity in plants by immunoblotting, in-gel assay, band shift, and immunoprecipitation.
    Article Snippet: Chemicals, peptides, and recombinant proteins Sodium hypochlorite (NaClO) Sinopharm Chemical Reagent CAS#: 7681-52-9 75% alcohol General reagent CAS#: G73537W Murashige & Skoog (MS) basal salt mixture Phyto Technology Cat#: M524 Sucrose Sinopharm Chemical Reagent CAS#: 57-50-1 Agar Sigma-Aldrich CAS#: 9002-18-0 NaCl Sinopharm Chemical Reagent CAS#: 7647-14-5 ABA Sinopharm Chemical Reagent CAS#: 14375-45-2 Mannitol Sinopharm Chemical Reagent CAS#: 69-65-8 Tris Sinopharm Chemical Reagent CAS#: 77-86-1 Sodium dodecyl sulfate (SDS) Sigma-Aldrich CAS#: 151-21-3 Glycine Sigma-Aldrich CAS#: 56-40-6 HEPES Sigma-Aldrich CAS#: 7365-45-9 EDTA Sinopharm Chemical Reagent CAS#: 60-00-4 EGTA Sigma-Aldrich CAS#: 67-42-5 Na3VO4 Sigma-Aldrich CAS#: 13721-39-6 NaF Sigma-Aldrich CAS#: 7681-49-4 β-glycerophosphate disodium Sigma-Aldrich CAS#: 154804-51-0 DTT Sigma-Aldrich CAS#: 3483-12-3 Leupeptin Sigma-Aldrich CAS#: 103476-89-7 Antipain Sigma-Aldrich Cat#: 10791 Aprotinin Roche Cat#: 10981532001 PMSF Sigma-Aldrich CAS#: 9036-06-0 HCl Sinopharm Chemical Reagent CAS#: 7647-01-0 MgCl2 Sigma-Aldrich CAS#: 7786-30-3 Histone type III-S Sigma-Aldrich CAS#: 9064-47-5 Triton X-100 Sigma-Aldrich CAS#: 9036-19-5 β-mercaptoethanol Sigma-Aldrich CAS#: 60-24-2 Color PAGE gel rapid preparation kit Epizyme Cat#: PG-112 Blue Plus IV protein marker TransGen Cat#: DM131-01 Trichloroacetic acid (TCA) Sigma-Aldrich CAS#: 76-03-9 Sodium pyrophosphate (NaPPi, Na4P2O7) Sigma-Aldrich CAS#: 13472-36-1 PVDF transfer membrane, 0.45 μm Invitrogen Cat#: 88518 Methanol Sinopharm Chemical Reagent Cat#: 67-56-1 Western blot fast stripping buffer Epizyme Cat#: PS107 Goat anti-rabbit IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1019 Anti-Myc tag (mouse monoclonal IgG1) Merck/Millipore Cat#: 05-724 Goat anti-mouse IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1011 ACTIN (N) antibody, rabbit polyclonal Abicode Cat#: R3772-1P Anti-GFP monoclonal antibody (JL-8) Takara Cat#: 632381 Anti-phospho-S175-SnRK2s antibody ABclonal Cat#: AP1481 Quick Start Bradford 1x dye reagent Bio-Rad Cat#: 5000205 Experimental models: Organisms/strains Arabidopsis thaliana: Col-0 N/A Arabidopsis thaliana: 35Spro:SnRK2.1-YFP in Col-0 background N/A Arabidopsis thaliana: SnRK2.4pro:SnRK2.4-GFP in snrk2.1/4/5/7/8/9/10 mutant background N/A Software and algorithms Amersham Typhoon scanner Amersham Typhoon N/A (Continued on next page) 2 STAR Protocols 6, 103842, June 20, 2025

    Marker:

    Article Title: Protocol for detecting SnRK2 kinase activity in plants by immunoblotting, in-gel assay, band shift, and immunoprecipitation.
    Article Snippet: Chemicals, peptides, and recombinant proteins Sodium hypochlorite (NaClO) Sinopharm Chemical Reagent CAS#: 7681-52-9 75% alcohol General reagent CAS#: G73537W Murashige & Skoog (MS) basal salt mixture Phyto Technology Cat#: M524 Sucrose Sinopharm Chemical Reagent CAS#: 57-50-1 Agar Sigma-Aldrich CAS#: 9002-18-0 NaCl Sinopharm Chemical Reagent CAS#: 7647-14-5 ABA Sinopharm Chemical Reagent CAS#: 14375-45-2 Mannitol Sinopharm Chemical Reagent CAS#: 69-65-8 Tris Sinopharm Chemical Reagent CAS#: 77-86-1 Sodium dodecyl sulfate (SDS) Sigma-Aldrich CAS#: 151-21-3 Glycine Sigma-Aldrich CAS#: 56-40-6 HEPES Sigma-Aldrich CAS#: 7365-45-9 EDTA Sinopharm Chemical Reagent CAS#: 60-00-4 EGTA Sigma-Aldrich CAS#: 67-42-5 Na3VO4 Sigma-Aldrich CAS#: 13721-39-6 NaF Sigma-Aldrich CAS#: 7681-49-4 β-glycerophosphate disodium Sigma-Aldrich CAS#: 154804-51-0 DTT Sigma-Aldrich CAS#: 3483-12-3 Leupeptin Sigma-Aldrich CAS#: 103476-89-7 Antipain Sigma-Aldrich Cat#: 10791 Aprotinin Roche Cat#: 10981532001 PMSF Sigma-Aldrich CAS#: 9036-06-0 HCl Sinopharm Chemical Reagent CAS#: 7647-01-0 MgCl2 Sigma-Aldrich CAS#: 7786-30-3 Histone type III-S Sigma-Aldrich CAS#: 9064-47-5 Triton X-100 Sigma-Aldrich CAS#: 9036-19-5 β-mercaptoethanol Sigma-Aldrich CAS#: 60-24-2 Color PAGE gel rapid preparation kit Epizyme Cat#: PG-112 Blue Plus IV protein marker TransGen Cat#: DM131-01 Trichloroacetic acid (TCA) Sigma-Aldrich CAS#: 76-03-9 Sodium pyrophosphate (NaPPi, Na4P2O7) Sigma-Aldrich CAS#: 13472-36-1 PVDF transfer membrane, 0.45 μm Invitrogen Cat#: 88518 Methanol Sinopharm Chemical Reagent Cat#: 67-56-1 Western blot fast stripping buffer Epizyme Cat#: PS107 Goat anti-rabbit IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1019 Anti-Myc tag (mouse monoclonal IgG1) Merck/Millipore Cat#: 05-724 Goat anti-mouse IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1011 ACTIN (N) antibody, rabbit polyclonal Abicode Cat#: R3772-1P Anti-GFP monoclonal antibody (JL-8) Takara Cat#: 632381 Anti-phospho-S175-SnRK2s antibody ABclonal Cat#: AP1481 Quick Start Bradford 1x dye reagent Bio-Rad Cat#: 5000205 Experimental models: Organisms/strains Arabidopsis thaliana: Col-0 N/A Arabidopsis thaliana: 35Spro:SnRK2.1-YFP in Col-0 background N/A Arabidopsis thaliana: SnRK2.4pro:SnRK2.4-GFP in snrk2.1/4/5/7/8/9/10 mutant background N/A Software and algorithms Amersham Typhoon scanner Amersham Typhoon N/A (Continued on next page) 2 STAR Protocols 6, 103842, June 20, 2025

    Membrane:

    Article Title: Protocol for detecting SnRK2 kinase activity in plants by immunoblotting, in-gel assay, band shift, and immunoprecipitation.
    Article Snippet: Chemicals, peptides, and recombinant proteins Sodium hypochlorite (NaClO) Sinopharm Chemical Reagent CAS#: 7681-52-9 75% alcohol General reagent CAS#: G73537W Murashige & Skoog (MS) basal salt mixture Phyto Technology Cat#: M524 Sucrose Sinopharm Chemical Reagent CAS#: 57-50-1 Agar Sigma-Aldrich CAS#: 9002-18-0 NaCl Sinopharm Chemical Reagent CAS#: 7647-14-5 ABA Sinopharm Chemical Reagent CAS#: 14375-45-2 Mannitol Sinopharm Chemical Reagent CAS#: 69-65-8 Tris Sinopharm Chemical Reagent CAS#: 77-86-1 Sodium dodecyl sulfate (SDS) Sigma-Aldrich CAS#: 151-21-3 Glycine Sigma-Aldrich CAS#: 56-40-6 HEPES Sigma-Aldrich CAS#: 7365-45-9 EDTA Sinopharm Chemical Reagent CAS#: 60-00-4 EGTA Sigma-Aldrich CAS#: 67-42-5 Na3VO4 Sigma-Aldrich CAS#: 13721-39-6 NaF Sigma-Aldrich CAS#: 7681-49-4 β-glycerophosphate disodium Sigma-Aldrich CAS#: 154804-51-0 DTT Sigma-Aldrich CAS#: 3483-12-3 Leupeptin Sigma-Aldrich CAS#: 103476-89-7 Antipain Sigma-Aldrich Cat#: 10791 Aprotinin Roche Cat#: 10981532001 PMSF Sigma-Aldrich CAS#: 9036-06-0 HCl Sinopharm Chemical Reagent CAS#: 7647-01-0 MgCl2 Sigma-Aldrich CAS#: 7786-30-3 Histone type III-S Sigma-Aldrich CAS#: 9064-47-5 Triton X-100 Sigma-Aldrich CAS#: 9036-19-5 β-mercaptoethanol Sigma-Aldrich CAS#: 60-24-2 Color PAGE gel rapid preparation kit Epizyme Cat#: PG-112 Blue Plus IV protein marker TransGen Cat#: DM131-01 Trichloroacetic acid (TCA) Sigma-Aldrich CAS#: 76-03-9 Sodium pyrophosphate (NaPPi, Na4P2O7) Sigma-Aldrich CAS#: 13472-36-1 PVDF transfer membrane, 0.45 μm Invitrogen Cat#: 88518 Methanol Sinopharm Chemical Reagent Cat#: 67-56-1 Western blot fast stripping buffer Epizyme Cat#: PS107 Goat anti-rabbit IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1019 Anti-Myc tag (mouse monoclonal IgG1) Merck/Millipore Cat#: 05-724 Goat anti-mouse IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1011 ACTIN (N) antibody, rabbit polyclonal Abicode Cat#: R3772-1P Anti-GFP monoclonal antibody (JL-8) Takara Cat#: 632381 Anti-phospho-S175-SnRK2s antibody ABclonal Cat#: AP1481 Quick Start Bradford 1x dye reagent Bio-Rad Cat#: 5000205 Experimental models: Organisms/strains Arabidopsis thaliana: Col-0 N/A Arabidopsis thaliana: 35Spro:SnRK2.1-YFP in Col-0 background N/A Arabidopsis thaliana: SnRK2.4pro:SnRK2.4-GFP in snrk2.1/4/5/7/8/9/10 mutant background N/A Software and algorithms Amersham Typhoon scanner Amersham Typhoon N/A (Continued on next page) 2 STAR Protocols 6, 103842, June 20, 2025

    Western Blot:

    Article Title: Protocol for detecting SnRK2 kinase activity in plants by immunoblotting, in-gel assay, band shift, and immunoprecipitation.
    Article Snippet: Chemicals, peptides, and recombinant proteins Sodium hypochlorite (NaClO) Sinopharm Chemical Reagent CAS#: 7681-52-9 75% alcohol General reagent CAS#: G73537W Murashige & Skoog (MS) basal salt mixture Phyto Technology Cat#: M524 Sucrose Sinopharm Chemical Reagent CAS#: 57-50-1 Agar Sigma-Aldrich CAS#: 9002-18-0 NaCl Sinopharm Chemical Reagent CAS#: 7647-14-5 ABA Sinopharm Chemical Reagent CAS#: 14375-45-2 Mannitol Sinopharm Chemical Reagent CAS#: 69-65-8 Tris Sinopharm Chemical Reagent CAS#: 77-86-1 Sodium dodecyl sulfate (SDS) Sigma-Aldrich CAS#: 151-21-3 Glycine Sigma-Aldrich CAS#: 56-40-6 HEPES Sigma-Aldrich CAS#: 7365-45-9 EDTA Sinopharm Chemical Reagent CAS#: 60-00-4 EGTA Sigma-Aldrich CAS#: 67-42-5 Na3VO4 Sigma-Aldrich CAS#: 13721-39-6 NaF Sigma-Aldrich CAS#: 7681-49-4 β-glycerophosphate disodium Sigma-Aldrich CAS#: 154804-51-0 DTT Sigma-Aldrich CAS#: 3483-12-3 Leupeptin Sigma-Aldrich CAS#: 103476-89-7 Antipain Sigma-Aldrich Cat#: 10791 Aprotinin Roche Cat#: 10981532001 PMSF Sigma-Aldrich CAS#: 9036-06-0 HCl Sinopharm Chemical Reagent CAS#: 7647-01-0 MgCl2 Sigma-Aldrich CAS#: 7786-30-3 Histone type III-S Sigma-Aldrich CAS#: 9064-47-5 Triton X-100 Sigma-Aldrich CAS#: 9036-19-5 β-mercaptoethanol Sigma-Aldrich CAS#: 60-24-2 Color PAGE gel rapid preparation kit Epizyme Cat#: PG-112 Blue Plus IV protein marker TransGen Cat#: DM131-01 Trichloroacetic acid (TCA) Sigma-Aldrich CAS#: 76-03-9 Sodium pyrophosphate (NaPPi, Na4P2O7) Sigma-Aldrich CAS#: 13472-36-1 PVDF transfer membrane, 0.45 μm Invitrogen Cat#: 88518 Methanol Sinopharm Chemical Reagent Cat#: 67-56-1 Western blot fast stripping buffer Epizyme Cat#: PS107 Goat anti-rabbit IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1019 Anti-Myc tag (mouse monoclonal IgG1) Merck/Millipore Cat#: 05-724 Goat anti-mouse IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1011 ACTIN (N) antibody, rabbit polyclonal Abicode Cat#: R3772-1P Anti-GFP monoclonal antibody (JL-8) Takara Cat#: 632381 Anti-phospho-S175-SnRK2s antibody ABclonal Cat#: AP1481 Quick Start Bradford 1x dye reagent Bio-Rad Cat#: 5000205 Experimental models: Organisms/strains Arabidopsis thaliana: Col-0 N/A Arabidopsis thaliana: 35Spro:SnRK2.1-YFP in Col-0 background N/A Arabidopsis thaliana: SnRK2.4pro:SnRK2.4-GFP in snrk2.1/4/5/7/8/9/10 mutant background N/A Software and algorithms Amersham Typhoon scanner Amersham Typhoon N/A (Continued on next page) 2 STAR Protocols 6, 103842, June 20, 2025

    Stripping Membranes:

    Article Title: Protocol for detecting SnRK2 kinase activity in plants by immunoblotting, in-gel assay, band shift, and immunoprecipitation.
    Article Snippet: Chemicals, peptides, and recombinant proteins Sodium hypochlorite (NaClO) Sinopharm Chemical Reagent CAS#: 7681-52-9 75% alcohol General reagent CAS#: G73537W Murashige & Skoog (MS) basal salt mixture Phyto Technology Cat#: M524 Sucrose Sinopharm Chemical Reagent CAS#: 57-50-1 Agar Sigma-Aldrich CAS#: 9002-18-0 NaCl Sinopharm Chemical Reagent CAS#: 7647-14-5 ABA Sinopharm Chemical Reagent CAS#: 14375-45-2 Mannitol Sinopharm Chemical Reagent CAS#: 69-65-8 Tris Sinopharm Chemical Reagent CAS#: 77-86-1 Sodium dodecyl sulfate (SDS) Sigma-Aldrich CAS#: 151-21-3 Glycine Sigma-Aldrich CAS#: 56-40-6 HEPES Sigma-Aldrich CAS#: 7365-45-9 EDTA Sinopharm Chemical Reagent CAS#: 60-00-4 EGTA Sigma-Aldrich CAS#: 67-42-5 Na3VO4 Sigma-Aldrich CAS#: 13721-39-6 NaF Sigma-Aldrich CAS#: 7681-49-4 β-glycerophosphate disodium Sigma-Aldrich CAS#: 154804-51-0 DTT Sigma-Aldrich CAS#: 3483-12-3 Leupeptin Sigma-Aldrich CAS#: 103476-89-7 Antipain Sigma-Aldrich Cat#: 10791 Aprotinin Roche Cat#: 10981532001 PMSF Sigma-Aldrich CAS#: 9036-06-0 HCl Sinopharm Chemical Reagent CAS#: 7647-01-0 MgCl2 Sigma-Aldrich CAS#: 7786-30-3 Histone type III-S Sigma-Aldrich CAS#: 9064-47-5 Triton X-100 Sigma-Aldrich CAS#: 9036-19-5 β-mercaptoethanol Sigma-Aldrich CAS#: 60-24-2 Color PAGE gel rapid preparation kit Epizyme Cat#: PG-112 Blue Plus IV protein marker TransGen Cat#: DM131-01 Trichloroacetic acid (TCA) Sigma-Aldrich CAS#: 76-03-9 Sodium pyrophosphate (NaPPi, Na4P2O7) Sigma-Aldrich CAS#: 13472-36-1 PVDF transfer membrane, 0.45 μm Invitrogen Cat#: 88518 Methanol Sinopharm Chemical Reagent Cat#: 67-56-1 Western blot fast stripping buffer Epizyme Cat#: PS107 Goat anti-rabbit IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1019 Anti-Myc tag (mouse monoclonal IgG1) Merck/Millipore Cat#: 05-724 Goat anti-mouse IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1011 ACTIN (N) antibody, rabbit polyclonal Abicode Cat#: R3772-1P Anti-GFP monoclonal antibody (JL-8) Takara Cat#: 632381 Anti-phospho-S175-SnRK2s antibody ABclonal Cat#: AP1481 Quick Start Bradford 1x dye reagent Bio-Rad Cat#: 5000205 Experimental models: Organisms/strains Arabidopsis thaliana: Col-0 N/A Arabidopsis thaliana: 35Spro:SnRK2.1-YFP in Col-0 background N/A Arabidopsis thaliana: SnRK2.4pro:SnRK2.4-GFP in snrk2.1/4/5/7/8/9/10 mutant background N/A Software and algorithms Amersham Typhoon scanner Amersham Typhoon N/A (Continued on next page) 2 STAR Protocols 6, 103842, June 20, 2025

    Enzyme-linked Immunosorbent Assay:

    Article Title: Protocol for detecting SnRK2 kinase activity in plants by immunoblotting, in-gel assay, band shift, and immunoprecipitation.
    Article Snippet: Chemicals, peptides, and recombinant proteins Sodium hypochlorite (NaClO) Sinopharm Chemical Reagent CAS#: 7681-52-9 75% alcohol General reagent CAS#: G73537W Murashige & Skoog (MS) basal salt mixture Phyto Technology Cat#: M524 Sucrose Sinopharm Chemical Reagent CAS#: 57-50-1 Agar Sigma-Aldrich CAS#: 9002-18-0 NaCl Sinopharm Chemical Reagent CAS#: 7647-14-5 ABA Sinopharm Chemical Reagent CAS#: 14375-45-2 Mannitol Sinopharm Chemical Reagent CAS#: 69-65-8 Tris Sinopharm Chemical Reagent CAS#: 77-86-1 Sodium dodecyl sulfate (SDS) Sigma-Aldrich CAS#: 151-21-3 Glycine Sigma-Aldrich CAS#: 56-40-6 HEPES Sigma-Aldrich CAS#: 7365-45-9 EDTA Sinopharm Chemical Reagent CAS#: 60-00-4 EGTA Sigma-Aldrich CAS#: 67-42-5 Na3VO4 Sigma-Aldrich CAS#: 13721-39-6 NaF Sigma-Aldrich CAS#: 7681-49-4 β-glycerophosphate disodium Sigma-Aldrich CAS#: 154804-51-0 DTT Sigma-Aldrich CAS#: 3483-12-3 Leupeptin Sigma-Aldrich CAS#: 103476-89-7 Antipain Sigma-Aldrich Cat#: 10791 Aprotinin Roche Cat#: 10981532001 PMSF Sigma-Aldrich CAS#: 9036-06-0 HCl Sinopharm Chemical Reagent CAS#: 7647-01-0 MgCl2 Sigma-Aldrich CAS#: 7786-30-3 Histone type III-S Sigma-Aldrich CAS#: 9064-47-5 Triton X-100 Sigma-Aldrich CAS#: 9036-19-5 β-mercaptoethanol Sigma-Aldrich CAS#: 60-24-2 Color PAGE gel rapid preparation kit Epizyme Cat#: PG-112 Blue Plus IV protein marker TransGen Cat#: DM131-01 Trichloroacetic acid (TCA) Sigma-Aldrich CAS#: 76-03-9 Sodium pyrophosphate (NaPPi, Na4P2O7) Sigma-Aldrich CAS#: 13472-36-1 PVDF transfer membrane, 0.45 μm Invitrogen Cat#: 88518 Methanol Sinopharm Chemical Reagent Cat#: 67-56-1 Western blot fast stripping buffer Epizyme Cat#: PS107 Goat anti-rabbit IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1019 Anti-Myc tag (mouse monoclonal IgG1) Merck/Millipore Cat#: 05-724 Goat anti-mouse IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1011 ACTIN (N) antibody, rabbit polyclonal Abicode Cat#: R3772-1P Anti-GFP monoclonal antibody (JL-8) Takara Cat#: 632381 Anti-phospho-S175-SnRK2s antibody ABclonal Cat#: AP1481 Quick Start Bradford 1x dye reagent Bio-Rad Cat#: 5000205 Experimental models: Organisms/strains Arabidopsis thaliana: Col-0 N/A Arabidopsis thaliana: 35Spro:SnRK2.1-YFP in Col-0 background N/A Arabidopsis thaliana: SnRK2.4pro:SnRK2.4-GFP in snrk2.1/4/5/7/8/9/10 mutant background N/A Software and algorithms Amersham Typhoon scanner Amersham Typhoon N/A (Continued on next page) 2 STAR Protocols 6, 103842, June 20, 2025

    Mutagenesis:

    Article Title: Protocol for detecting SnRK2 kinase activity in plants by immunoblotting, in-gel assay, band shift, and immunoprecipitation.
    Article Snippet: Chemicals, peptides, and recombinant proteins Sodium hypochlorite (NaClO) Sinopharm Chemical Reagent CAS#: 7681-52-9 75% alcohol General reagent CAS#: G73537W Murashige & Skoog (MS) basal salt mixture Phyto Technology Cat#: M524 Sucrose Sinopharm Chemical Reagent CAS#: 57-50-1 Agar Sigma-Aldrich CAS#: 9002-18-0 NaCl Sinopharm Chemical Reagent CAS#: 7647-14-5 ABA Sinopharm Chemical Reagent CAS#: 14375-45-2 Mannitol Sinopharm Chemical Reagent CAS#: 69-65-8 Tris Sinopharm Chemical Reagent CAS#: 77-86-1 Sodium dodecyl sulfate (SDS) Sigma-Aldrich CAS#: 151-21-3 Glycine Sigma-Aldrich CAS#: 56-40-6 HEPES Sigma-Aldrich CAS#: 7365-45-9 EDTA Sinopharm Chemical Reagent CAS#: 60-00-4 EGTA Sigma-Aldrich CAS#: 67-42-5 Na3VO4 Sigma-Aldrich CAS#: 13721-39-6 NaF Sigma-Aldrich CAS#: 7681-49-4 β-glycerophosphate disodium Sigma-Aldrich CAS#: 154804-51-0 DTT Sigma-Aldrich CAS#: 3483-12-3 Leupeptin Sigma-Aldrich CAS#: 103476-89-7 Antipain Sigma-Aldrich Cat#: 10791 Aprotinin Roche Cat#: 10981532001 PMSF Sigma-Aldrich CAS#: 9036-06-0 HCl Sinopharm Chemical Reagent CAS#: 7647-01-0 MgCl2 Sigma-Aldrich CAS#: 7786-30-3 Histone type III-S Sigma-Aldrich CAS#: 9064-47-5 Triton X-100 Sigma-Aldrich CAS#: 9036-19-5 β-mercaptoethanol Sigma-Aldrich CAS#: 60-24-2 Color PAGE gel rapid preparation kit Epizyme Cat#: PG-112 Blue Plus IV protein marker TransGen Cat#: DM131-01 Trichloroacetic acid (TCA) Sigma-Aldrich CAS#: 76-03-9 Sodium pyrophosphate (NaPPi, Na4P2O7) Sigma-Aldrich CAS#: 13472-36-1 PVDF transfer membrane, 0.45 μm Invitrogen Cat#: 88518 Methanol Sinopharm Chemical Reagent Cat#: 67-56-1 Western blot fast stripping buffer Epizyme Cat#: PS107 Goat anti-rabbit IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1019 Anti-Myc tag (mouse monoclonal IgG1) Merck/Millipore Cat#: 05-724 Goat anti-mouse IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1011 ACTIN (N) antibody, rabbit polyclonal Abicode Cat#: R3772-1P Anti-GFP monoclonal antibody (JL-8) Takara Cat#: 632381 Anti-phospho-S175-SnRK2s antibody ABclonal Cat#: AP1481 Quick Start Bradford 1x dye reagent Bio-Rad Cat#: 5000205 Experimental models: Organisms/strains Arabidopsis thaliana: Col-0 N/A Arabidopsis thaliana: 35Spro:SnRK2.1-YFP in Col-0 background N/A Arabidopsis thaliana: SnRK2.4pro:SnRK2.4-GFP in snrk2.1/4/5/7/8/9/10 mutant background N/A Software and algorithms Amersham Typhoon scanner Amersham Typhoon N/A (Continued on next page) 2 STAR Protocols 6, 103842, June 20, 2025

    Software:

    Article Title: Protocol for detecting SnRK2 kinase activity in plants by immunoblotting, in-gel assay, band shift, and immunoprecipitation.
    Article Snippet: Chemicals, peptides, and recombinant proteins Sodium hypochlorite (NaClO) Sinopharm Chemical Reagent CAS#: 7681-52-9 75% alcohol General reagent CAS#: G73537W Murashige & Skoog (MS) basal salt mixture Phyto Technology Cat#: M524 Sucrose Sinopharm Chemical Reagent CAS#: 57-50-1 Agar Sigma-Aldrich CAS#: 9002-18-0 NaCl Sinopharm Chemical Reagent CAS#: 7647-14-5 ABA Sinopharm Chemical Reagent CAS#: 14375-45-2 Mannitol Sinopharm Chemical Reagent CAS#: 69-65-8 Tris Sinopharm Chemical Reagent CAS#: 77-86-1 Sodium dodecyl sulfate (SDS) Sigma-Aldrich CAS#: 151-21-3 Glycine Sigma-Aldrich CAS#: 56-40-6 HEPES Sigma-Aldrich CAS#: 7365-45-9 EDTA Sinopharm Chemical Reagent CAS#: 60-00-4 EGTA Sigma-Aldrich CAS#: 67-42-5 Na3VO4 Sigma-Aldrich CAS#: 13721-39-6 NaF Sigma-Aldrich CAS#: 7681-49-4 β-glycerophosphate disodium Sigma-Aldrich CAS#: 154804-51-0 DTT Sigma-Aldrich CAS#: 3483-12-3 Leupeptin Sigma-Aldrich CAS#: 103476-89-7 Antipain Sigma-Aldrich Cat#: 10791 Aprotinin Roche Cat#: 10981532001 PMSF Sigma-Aldrich CAS#: 9036-06-0 HCl Sinopharm Chemical Reagent CAS#: 7647-01-0 MgCl2 Sigma-Aldrich CAS#: 7786-30-3 Histone type III-S Sigma-Aldrich CAS#: 9064-47-5 Triton X-100 Sigma-Aldrich CAS#: 9036-19-5 β-mercaptoethanol Sigma-Aldrich CAS#: 60-24-2 Color PAGE gel rapid preparation kit Epizyme Cat#: PG-112 Blue Plus IV protein marker TransGen Cat#: DM131-01 Trichloroacetic acid (TCA) Sigma-Aldrich CAS#: 76-03-9 Sodium pyrophosphate (NaPPi, Na4P2O7) Sigma-Aldrich CAS#: 13472-36-1 PVDF transfer membrane, 0.45 μm Invitrogen Cat#: 88518 Methanol Sinopharm Chemical Reagent Cat#: 67-56-1 Western blot fast stripping buffer Epizyme Cat#: PS107 Goat anti-rabbit IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1019 Anti-Myc tag (mouse monoclonal IgG1) Merck/Millipore Cat#: 05-724 Goat anti-mouse IgG (H+L)-HRP conjugate, EIA Bio-Rad Cat#: 172-1011 ACTIN (N) antibody, rabbit polyclonal Abicode Cat#: R3772-1P Anti-GFP monoclonal antibody (JL-8) Takara Cat#: 632381 Anti-phospho-S175-SnRK2s antibody ABclonal Cat#: AP1481 Quick Start Bradford 1x dye reagent Bio-Rad Cat#: 5000205 Experimental models: Organisms/strains Arabidopsis thaliana: Col-0 N/A Arabidopsis thaliana: 35Spro:SnRK2.1-YFP in Col-0 background N/A Arabidopsis thaliana: SnRK2.4pro:SnRK2.4-GFP in snrk2.1/4/5/7/8/9/10 mutant background N/A Software and algorithms Amersham Typhoon scanner Amersham Typhoon N/A (Continued on next page) 2 STAR Protocols 6, 103842, June 20, 2025



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    Bio-Rad 172 1011 actin n antibody
    172 1011 Actin N Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/172+1011+actin+n+antibody/Goat+Anti-Mouse+IgG+(H+%2B+L)-HRP+Conjugate/pm40440176-24-224-251
    Average 99 stars, based on 1 article reviews
    172 1011 actin n antibody - by Bioz Stars, 2026-09
    99/100 stars
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